![]() ![]() Its His-289 residue in the re-side of the isoalloxazine ring is within hydrogen bonding distance with a hydroxyl group of 2,5-DiCHQ. The structure of TftD was also similar to the known structures of FADH 2-dependent monooxygenases. The stacked nicotinamide:isoalloxazine rings in TftC and sequential reaction kinetics suggest that the reduced FAD leaves TftC after NADH oxidation. The structure of TftC was similar to those of related flavin reductases. We have determined the crystal structures of dimeric TftC and tetrameric TftD at 2.0 and 2.5 Å resolution, respectively. In those processes, TftC provides all the required FADH 2. Then, TftD oxidizes the latter to 5-chloro-2-hydroxy -p-benzoquinone. TftD oxidizes 2,4,5-trichlorophenol (2,4,5-TCP) to 2,5-dichloro- p-benzoquinone, which is chemically reduced to 2,5-dichloro- p-hydroquinone (2,5-DiCHQ). It does not store any personal data.Burkholderia cepacia AC1100 completely degrades 2,4,5-trichlorophenol, in which an FADH 2-dependent monooxygenase (TftD) and an NADH:FAD oxidoreductase (TftC) catalyze the initial steps. The cookie is set by the GDPR Cookie Consent plugin and is used to store whether or not user has consented to the use of cookies. The cookie is used to store the user consent for the cookies in the category "Performance". This cookie is set by GDPR Cookie Consent plugin. The cookie is used to store the user consent for the cookies in the category "Other. The cookies is used to store the user consent for the cookies in the category "Necessary". ![]() The cookie is set by GDPR cookie consent to record the user consent for the cookies in the category "Functional". The cookie is used to store the user consent for the cookies in the category "Analytics". ![]() These cookies ensure basic functionalities and security features of the website, anonymously. Necessary cookies are absolutely essential for the website to function properly. ![]()
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